analysis of variance (anova) and tukey’s test using graphpad prism 5  (GraphPad Software Inc)

Journal: Journal of Fungi

Article Title: Differential Physiological Prerequisites and Gene Expression Profiles of Conidial Anastomosis Tube and Germ Tube Formation in Colletotrichum gloeosporioides

doi: 10.3390/jof7070509

Figure Lengend Snippet: Germ tube formation and conidial anastomosis tube fusion dynamics in C. gloeosporioides . ( A ) Percentage GT/CAT formation in different aged conidia, viz., 6, 10, 13, and 17 days of C. gloeosporioides . ( B ) Percentage CAT fusion in 17 days old conidia of C. gloeosporioides incubated for different incubation times, viz., 24, 48, 72, and 96 h. ( C ) Percentage GT formation in 6 days old conidia of C. gloeosporioides incubated for different incubation times, viz., 3, 6, 12, 18, and 24 h. ( D ) Percentage CAT fusion in different conidial densities, viz., 4 × 10 2 , 4 × 10 3 , 4 × 10 4 , 4 × 10 5 , and 4 × 10 6 of 17-day-old conidia incubated in 1 mL of water. ( E ) Percentage GT formation in different conidial densities, viz., 4 × 10 2 , 4 × 10 3 , 4 × 10 4 , 4 × 10 5 , and 4 × 10 6 of 6-day-old conidia incubated in 1 mL of PDB. ( F ) CAT and GT formation percentage in PDB, water, glucose, and Insolution ™ PD98059 (inhibitor of MEK), CAT fusion induced in water supplemented with glucose and Insolution ™ PD98059; GT formation was assayed in PDB supplemented with glucose and Insolution ™ PD98059. GT/CAT formation was quantified as the percentage of conidia involved in GT/CAT formation. Average from three replicates ( n = 3), and 300 conidial numbers were counted per replicate. Bar indicates standard deviation. Statistical significance of differences was analyzed by one-way ANOVA with Tukey’s multiple comparison post-hoc test (bars with the same letter are not significantly different; p ≤ 0.05).

Article Snippet: The GT formation and CAT fusion data were analyzed by Analysis of Variance (ANOVA) with Tukey’s post hoc test using GraphPad Prism 5 Statistics Software.

Techniques: Incubation, Standard Deviation, Comparison

Journal: Applied and Environmental Microbiology

Article Title: Molecular and Functional Analysis of the Type IV Pilus Gene Cluster in Streptococcus sanguinis SK36

doi: 10.1128/AEM.02788-18

Figure Lengend Snippet: CAT-specific activities of the recombinant S. sanguinis strains harboring a wild-type pilB promoter-cat translational fusion or fusions driven by the derivatives of the promoter region. The relative locations of the three promoters are shown at the top. The name of the fusion strain and its genotype are listed to the left of the graph describing CAT-specific activity. The location of spe in the fusion is indicated by an inverted triangle. The putative −10 element was replaced with 5′-CTGCAG (open boxes) and 5′-CTCGAC (filled boxes) by site-directed mutagenesis. All strains were harvested at an OD600 of 0.8. Values are the means and standard deviations from three independent experiments. Significant differences between the fusion of the wild-type promoter and fusions of the mutated promoters were analyzed using one-way ANOVA followed by a Dunnett test. *, P < 0.01.

Article Snippet: Statistical analysis was performed using analysis of variance (ANOVA) followed by a Dunnett or Tukey test using GraphPad Prism 5.

Techniques: Recombinant, Activity Assay, Mutagenesis

Journal: Applied and Environmental Microbiology

Article Title: Molecular and Functional Analysis of the Type IV Pilus Gene Cluster in Streptococcus sanguinis SK36

doi: 10.1128/AEM.02788-18

Figure Lengend Snippet: Biofilm formation of S. sanguinis SK36 and its derivatives. The final growth yield was measured at 490 nm (open bars), and the biofilm mass was quantified at 562 nm (filled bars). The values are the means and standard deviations from three independent experiments. Significant differences between wild-type SK36 and its derivatives were analyzed using one-way ANOVA followed by a Dunnett test. ***, P < 0.0001.

Article Snippet: Statistical analysis was performed using analysis of variance (ANOVA) followed by a Dunnett or Tukey test using GraphPad Prism 5.

Techniques: